DISTRIBUTION OF MOLECULAR AGGREGATION BY ANALYSIS OF FLUCTUATION MOMENTS

被引：143

作者：

QIAN, H ^{[1
]}

ELSON, EL ^{[1
]}

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT BIOCHEM & MOLEC BIOPHYS,ST LOUIS,MO 63110

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 14期

关键词：

Compound Poisson distribution; Fluorescence spectroscopy; Occupation number; Random emission; Shot noise;

D O I：

10.1073/pnas.87.14.5479

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The fluorescence from an open volume of a solution of fluorescent molecules fluctuates as the molecules randomly diffuse into and out of the volume. The distribution of degrees of aggregation or polymerization of the fluorescent molecules can be characterized without perturbing the system by measuring either the moments or the amplitude distribution of these fluctuations. We present an experimental verification of this approach applied to simple model systems consisting of solutions of fluorescent particles of well-defined size. We have also characterized the response of the photon-detection device (typically a photomultiplier), which is essential to the analysis of the fluorescence fluctuations, and have compared two methods for determining shot-noise contributions.

引用

页码：5479 / 5483

页数：5