HIGH-LEVEL EXPRESSION OF ACTIVE HUMAN ALPHA-1-ANTITRYPSIN IN THE MILK OF TRANSGENIC SHEEP

被引：282

作者：

WRIGHT, G

CARVER, A

COTTOM, D

REEVES, D

SCOTT, A

SIMONS, P

WILMUT, I

GARNER, I

COLMAN, A

机构：

[1] PHARMACEUT PROT LTD,KINGS BLDG,W MAINS RD,EDINBURGH EH9 3JQ,SCOTLAND

[2] AFRC,INST ANIM PHYSIOL & GENET RES,EDINBURGH RES STN,EDINBURGH EH9 3JQ,MIDLOTHIAN,SCOTLAND

来源：

BIO-TECHNOLOGY | 1991年 / 9卷 / 09期

关键词：

D O I：

10.1038/nbt0991-830

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe the generation of five sheep transgenic for a fusion of the ovine beta-lactoglobulin gene promotor to the human alpha(1)-antitrypsin (h-alpha(1)AT) genomic sequences. Four of these animals are female and one male. Analysis of the expression of h-alpha(1)AT in the milk of three of these females shows that all express the human protein at levels greater than 1 gram per liter. In one case initial levels exceeded 60 grams per liter and stabilized at approximately 35 grams per liter as lactation progressed. Human alpha(1)AT purified from the milk of these animals appears to be fully N-glycosylated and has a biological activity indistinguishable from human plasma-derived material.

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页码：830 / 834

页数：5

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