EXPRESSION OF RECOMBINANT PLATELET-DERIVED ENDOTHELIAL-CELL GROWTH-FACTOR IN THE YEAST SACCHAROMYCES-CEREVISIAE

被引：6

作者：

FINNIS, C

GOODEY, A

COURTNEY, M

SLEEP, D

机构：

[1] Delta Biotechnology Ltd, Nottingham, NG7 1FD, Castle Court

来源：

YEAST | 1992年 / 8卷 / 01期

关键词：

SACCHAROMYCES-CEREVISIAE; PLATELET-DERIVED ENDOTHELIAL CELL GROWTH FACTOR;

D O I：

10.1002/yea.320080106

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A platelet-derived endothelial cell growth factor cDNA has been cloned, sequenced and expressed using the Saccharomyces cerevisiae PRB1 promoter. Soluble recombinant platelet-derived endothelial cell growth factor constituted 0.5-1.0% of total soluble protein. Yeast soluble protein extracts containing recombinant platelet-derived endothelial cell growth factor stimulate the growth of calf pulmonary artery endothelial cells in vitro.

引用

页码：57 / 60

页数：4

共 12 条

[1] TRANSFORMATION OF YEAST BY A REPLICATING HYBRID PLASMID
BEGGS, JD
[J]. NATURE, 1978, 275 (5676) : 104 - 109
[2] BEGGS JD, 1981, A BENZON S, V16, P383
[3] FRESHNEY RI, 1987, CULTURE ANIMAL CELLS, P83
[4] ORGANIZATION AND CHROMOSOMAL LOCALIZATION OF THE HUMAN PLATELET-DERIVED ENDOTHELIAL-CELL GROWTH-FACTOR GENE
HAGIWARA, K
STENMAN, G
HONDA, H
SAHLIN, P
ANDERSSON, A
MIYAZONO, K
HELDIN, CH
ISHIKAWA, F
TAKAKU, F
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (04) : 2125 - 2132
[5] IDENTIFICATION OF ANGIOGENIC ACTIVITY AND THE CLONING AND EXPRESSION OF PLATELET-DERIVED ENDOTHELIAL-CELL GROWTH-FACTOR
ISHIKAWA, F
MIYAZONO, K
HELLMAN, U
DREXLER, H
WERNSTEDT, C
HAGIWARA, K
USUKI, K
TAKAKU, F
RISAU, W
HELDIN, CH
[J]. NATURE, 1989, 338 (6216) : 557 - 562
[6] HIGH-YIELD PURIFICATION OF PLATELET-DERIVED ENDOTHELIAL-CELL GROWTH-FACTOR - STRUCTURAL CHARACTERIZATION AND ESTABLISHMENT OF A SPECIFIC ANTISERUM
MIYAZONO, K
HELDIN, CH
[J]. BIOCHEMISTRY, 1989, 28 (04) : 1704 - 1710
[7] MIYAZONO K, 1987, J BIOL CHEM, V262, P4098
[8] A PLATELET FACTOR THAT STIMULATES THE PROLIFERATION OF VASCULAR ENDOTHELIAL-CELLS
MIYAZONO, K
OKABE, T
URABE, A
YAMANAKA, M
TAKAKU, F
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 126 (01) : 83 - 88
[9] SHERMAN F, 1986, LABORATORY COURSE MA
[10] SACCHAROMYCES-CEREVISIAE STRAINS THAT OVEREXPRESS HETEROLOGOUS PROTEINS
SLEEP, D
BELFIELD, GP
BALLANCE, DJ
STEVEN, J
JONES, S
EVANS, LR
MOIR, PD
GOODEY, AR
[J]. BIO-TECHNOLOGY, 1991, 9 (02): : 183 - 187

← 1 2 →