TRANSDUCTION OF THE LIGHT SIGNAL DURING COMPLEMENTARY CHROMATIC ADAPTATION IN THE CYANOBACTERIUM CALOTHRIX SP PCC-7601 - DNA-BINDING PROTEINS AND MODULATION BY PHOSPHORYLATION

被引：55

作者：

SOBCZYK, A ^{[1
]}

SCHYNS, G ^{[1
]}

DEMARSAC, NT ^{[1
]}

HOUMARD, J ^{[1
]}

机构：

[1] INST PASTEUR,DEPT BIOCHIM & GENET MOLEC,CNRS,URA 1129,28 RUE DR ROUX,F-75724 PARIS 15,FRANCE

来源：

EMBO JOURNAL | 1993年 / 12卷 / 03期

关键词：

PHOTOREGULATION; PHYCOERYTHRIN; POSTTRANSLATIONAL MODIFICATION; RCAA AND RCAB; TRANSCRIPTION FACTORS;

D O I：

10.1002/j.1460-2075.1993.tb05740.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cyanobacterium Calothrix sp. PCC 7601 can adapt its pigment content in response to changes in the incident light wavelength. It synthesizes, as major tight-harvesting pigments, either phycocyanin 2 (PC2, encoded by the cpc2 operon) under red tight or phycoerythrin (PE, encoded by the cpeBA operon) under green light conditions. The last step of the signal transduction pathway is characterized by a transcriptional control of the expression of these operons. Partially purified protein extracts were used in gel retardation assays and DNase I footprinting experiments to identify the factors that interact with the promoter region of the cpeBA operon. We found that two proteins, RcaA and RcaB, only detected in extracts of cells grown under green light, behave as positive transcriptional factors for the expression of the cpeBA operon. Treatment of the fractions containing RcaA and RcaB with alkaline phosphatase prevents the binding of RcaA but not of RcaB to the cpeBA promoter region. A post-translational modification of RcaA thus modulates its affinity for DNA.

引用

页码：997 / 1004

页数：8

共 45 条

[21] A GEL-ELECTROPHORESIS METHOD FOR QUANTIFYING THE BINDING OF PROTEINS TO SPECIFIC DNA REGIONS - APPLICATION TO COMPONENTS OF THE ESCHERICHIA-COLI LACTOSE OPERON REGULATORY SYSTEM [J].

GARNER, MM ;

REVZIN, A .

NUCLEIC ACIDS RESEARCH, 1981, 9 (13) :3047-3060

[22] 3 C-PHYCOERYTHRIN-ASSOCIATED LINKER POLYPEPTIDES IN THE PHYCOBILISOME OF GREEN-LIGHT-GROWN CALOTHRIX SP PCC 7601 (CYANOBACTERIA) [J].

GLAUSER, M ;

SIDLER, WA ;

GRAHAM, KW ;

BRYANT, DA ;

FRANK, G ;

WEHRLI, E ;

ZUBER, H .

FEBS LETTERS, 1992, 297 (1-2) :19-23

[23] FAMILIES OF BACTERIAL SIGNAL-TRANSDUCING PROTEINS [J].

GROSS, R ;

ARICO, B ;

RAPPUOLI, R .

MOLECULAR MICROBIOLOGY, 1989, 3 (11) :1661-1667

[24] CHROMATIC ADAPTATION AND THE EVENTS INVOLVED IN PHYCOBILISOME BIOSYNTHESIS [J].

GROSSMAN, AR .

PLANT CELL AND ENVIRONMENT, 1990, 13 (07) :651-666

[25] ACTION SPECTRA FOR PHYCOBILIPROTEIN SYNTHESIS IN A CHROMATICALLY ADAPTING CYANOPHYTE, FREMYELLA-DIPLOSIPHON [J].

HAURY, JF ;

BOGORAD, L .

PLANT PHYSIOLOGY, 1977, 60 (06) :835-839

[26]

HEERY DM, 1990, TRENDS GENET, V6, P173

[27] UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING [J].

HENIKOFF, S .

GENE, 1984, 28 (03) :351-359

[28] CROSSTALK - A PIVOTAL ROLE FOR PROTEIN-KINASE-C IN MODULATING RELATIONSHIPS BETWEEN SIGNAL TRANSDUCTION PATHWAYS [J].

HOUSLAY, MD .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 195 (01) :9-27

[29] TRANSCRIPTIONAL ORGANIZATION OF THE PHYCOCYANIN SUBUNIT GENE CLUSTERS OF THE CYANOBACTERIUM ANACYSTIS-NIDULANS UTEX-625 [J].

KALLA, SR ;

LIND, LK ;

LIDHOLM, J ;

GUSTAFSSON, P .

JOURNAL OF BACTERIOLOGY, 1988, 170 (07) :2961-2970

[30]

KREBS EG, 1986, ENZYMES, V17, P3

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