PRECISE MAPPING AND MOLECULAR CHARACTERIZATION OF THE MFT1 GENE INVOLVED IN IMPORT OF A FUSION PROTEIN INTO MITOCHONDRIA IN SACCHAROMYCES-CEREVISIAE

Garrett et al. [Mol. Gen. Genet. 225 (1991) 483-491] recently reported that an Atp2-lacZ fusion protein was transported into mitochondria in yeast, thus identifying the MFT1 (mitochondrial fusion targeting) gene as a genomic fragment which complements a mutation (mft1) that failed in targeting a fusion protein into mitochondria [4]. They mapped this gene to the ORF, which we have independently identified as a gene homologous to the cyc07 gene, which is expressed specifically in the S phase during the plant cell cycle. We have mapped the MFT1 gene precisely and found that this gene should correspond to the neighboring ORF, rather than the ORF they identified.