REGULATION OF PROLIFERATION-SPECIFIC AND DIFFERENTIATION-SPECIFIC GENES DURING SENESCENCE OF HUMAN EPIDERMAL KERATINOCYTE AND MAMMARY EPITHELIAL-CELLS

被引：53

作者：

SAUNDERS, NA ^{[1
]}

SMITH, RJ ^{[1
]}

JETTEN, AM ^{[1
]}

机构：

[1] NIEHS,CELL BIOL SECT,PULM PATHOBIOL LAB,POB 12233,RES TRIANGLE PK,NC 27709

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 197卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.2439

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of proliferation-associated genes, cdc2 and E2F-1 and squamous-specific genes transglutaminase type I and cornifin were examined in senescing human epidermal keratinocytes. Cultured keratinocytes underwent 34 population doublings before senescing. Senescence in keratinocytes was characterised by reduced thymidine incorporation, a change in morphology and the inability of cells to undergo mitosis. This was accompanied by downregulation of cdc2 and E2F-1 mRNA's. In addition, senescing keratinocytes started to express genes such as cornifin, that are specific for squamous differentiation. These changes were similar to those observed in keratinocytes induced to differentiate with phorbol ester or by confluence. E2F-1, cdc2 and cornifin were similarly altered in senescing human mammary epithelial cells. Our data suggest that events regulating senescence may also be linked to squamous differentiation. © 1993 Academic Press, Inc.

引用

页码：46 / 54

页数：9

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