CAROTENOID AND STEROID SYNTHESES BY CARROT CELLS IN SUSPENSION CULTURE

被引:14
作者
SHIMIZU, K [1 ]
KIKUCHI, T [1 ]
SUGANO, N [1 ]
NISHI, A [1 ]
机构
[1] UNIV TOYAMA,WAKANYAKU RES INST,TOYAMA,JAPAN
关键词
D O I
10.1111/j.1399-3054.1979.tb06544.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Biosynthesis of carotenoids and steroids in tissue cultures of carrot cells I Daucus carota L.) was studied by measuring the change in their contents and incorporation of 14lC‐acelate. The rate of synthesis of these plant products varied significantly during the growth cycle in batch culture. Carotenoids were most actively synthesized in the early logarithmic phase and the synthetic rate sharply declined as the culture aged, whereas the rate of accumulation of phytosterols was highest at the end of the growth phase. Newly synthesized carotenes were shown to undergo turnover during the growth. The synthesis of both carotecnoid and steroid was stimulated by 2.‐ 4‐dichlorophenoxyacetic acid. Copyright © 1979, Wiley Blackwell. All rights reserved
引用
收藏
页码:127 / 132
页数:6
相关论文
共 13 条
[1]  
DAVIES BH, 1976, CHEMISTRY BIOCHEMIST, V2, P38, DOI DOI 10.1590/S0101-20612001000200017
[2]   PLANT TISSUE CULTURES .6. REGULATION OF NICOTINE BIOSYNTHESIS BY AUXINS IN TOBACCO CALLUS TISSUES [J].
FURUYA, T ;
KOJIMA, H ;
SYONO, K .
PHYTOCHEMISTRY, 1971, 10 (07) :1529-&
[3]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[4]  
MOK MC, 1976, J AM SOC HORTIC SCI, V101, P442
[5]   A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].
MURASHIGE, T ;
SKOOG, F .
PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497
[6]  
NISHI A, 1974, PHYTOCHEMISTRY, V13, P1653, DOI 10.1016/0031-9422(74)85069-7
[7]   PHYSIOLOGICAL CHANGES OF CARROT CELLS IN SUSPENSION CULTURE DURING GROWTH AND SENESCENCE [J].
OKAMURA, S ;
SUEKI, K ;
NISHI, A .
PHYSIOLOGIA PLANTARUM, 1975, 33 (04) :251-255
[8]   METABOLISM AND FUNCTION OF ALKALOIDS IN PLANTS [J].
ROBINSON, T .
SCIENCE, 1974, 184 (4135) :430-435
[9]   FORMATION OF PHENOLIC ACID IN CARROT CELLS IN SUSPENSION CULTURES [J].
SUGANO, N ;
IWATA, R ;
NISHI, A .
PHYTOCHEMISTRY, 1975, 14 (5-6) :1205-1207
[10]  
SUGANO N, 1971, PLANT CELL PHYSIOL, V12, P525