A NEW SCREENING METHOD FOR GLUCOSIDASE INHIBITORS AND ITS APPLICATION TO ALGAL EXTRACTS

被引：13

作者：

KURIHARA, H

SASAKI, M

HATANO, M

机构：

[1] Department of Food Science and Technology, Faculty of Fisheries, Hokkaido University, Minato, Hakodate

来源：

FISHERIES SCIENCE | 1994年 / 60卷 / 06期

关键词：

SCREENING; GLUCOSIDASE; INHIBITOR; ALGAE;

D O I：

10.2331/fishsci.60.759

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

A screening method for alpha- and beta-D-glucosidase inhibitors was developed by using agar plates. An enzyme agar plate containing either alpha- or beta-D-glucosidase was used in combination with a substrate agar plate containing p-nitrophenyl alpha- or beta-D-glucopyranoside. Paper disks impregnated with algal extracts were placed on the enzyme agar plate, then preincubated for 2.5 h at 25-degrees-C. The substrate agar was layered on the enzyme agar. Inhibitory zones appeared colorless in the yellow background of p-nitrophenol. Extracts of algae collected in southern Hokkaido were screened for alpha- and beta-D-glucosidase inhibitors by the proposed agar plate method. Among nine algal species examined, seven species inhibited alpha-D-glucosidase, where five species inhibited beta-D-glucosidase.

引用

页码：759 / 761

页数：3

共 14 条

[1] SYNTHESIS AND BIOLOGICAL-ACTIVITY OF C-6 MODIFIED DERIVATIVES OF THE GLUCOSIDASE INHIBITOR 1-DEOXYNOJIRIMYCIN [J].