EFFECT OF FENARIMOL ON STEROL BIOSYNTHESIS IN SUSPENSION CULTURES OF BRAMBLE CELLS

被引：52

作者：

SCHMITT, P

BENVENISTE, P

机构：

[1] Institut de Botanique, Laboratoire de Biochimie Végétale, E.R.A. du C.N.R.S. No. 487, 67087 Strasbourg Cedex, 28, rue Goethe

来源：

PHYTOCHEMISTRY | 1979年 / 18卷 / 10期

关键词：

14α-methyl demethylation; !sup]1[!/sup]H NMR of Δ[!sup]8[!/sup]-sterols; fenarimol; Rosaceae; Rubus fruticosus; sterol biosynthesis inhibitor;

D O I：

10.1016/0031-9422(79)80179-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bramble suspension cultures normally contain Δ5-sterols (sitosterol, campesterol and isofucosterol). When the cells were grown in a medium supplemented with fenarimol, 14α-methyl sterols accumulated. Eight 14α-methyl sterols, including two new compounds, 4α,14α-dimethyl-stigmasta-8,Z-24(28)-dien-3β-ol and 14α-methyl-stigmasta-8,Z-24(28)-dien-3β-ol, were identified. Fenarimol probably inhibited the 14α-methyl demethylation. Cell lines growing permanently in 2 fenarimol-supplemented medium were obtained. © 1979.

引用

页码：1659 / 1665

页数：7

共 26 条

[1] 14-ALPHA-DEMETHYLATION OF LANOSTEROL BY A RECONSTITUTED CYTOCHROME-P-450 SYSTEM FROM YEAST MICROSOMES [J].

AOYAMA, Y ;

YOSHIDA, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1978, 85 (01) :28-34

[2] INVESTIGATIONS ON BIOSYNTHESIS OF STEROIDS AND TERPENOIDS .2. ROLE OF 24-METHYLENE DERIVATIVES IN BIOSYNTHESIS OF STERIODS AND TERPENOIDS [J].

BARTON, DHR ;

HARRISON, DM ;

MOSS, GP ;

WIDDOWSON, DA .

JOURNAL OF THE CHEMICAL SOCIETY C-ORGANIC, 1970, (06) :775-+

[3]

BATES RB, 1968, TETRAHEDRON LETT, P6163

[4] STEROLS IN PORPHYRIDIUM SPECIES - 4ALPHA-METHYL-5ALPHA-CHOLESTA-8,22-DIEN-3BETA-OL AND 4ALPHA,24XI-DIMETHYL-5ALPHA-CHOLESTA-8,22-DIEN-3B2NOVEL STEROLS FROM PORPHYRIDIUM-CRUENTUM [J].

BEASTALL, GH ;

TYNDALL, AM ;

REES, HH ;

GOODWIN, TW .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 41 (02) :301-309

[5]

BHACCA NS, 1964, APPLICATIONS NMR SPE, P108

[6] FUNCTION OF STEROLS IN MEMBRANES [J].

DEMEL, RA ;

DEKRUYFF, B .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 457 (02) :109-132

[7] 14ALPHA-METHYL-5ALPHA-ERGOST-8-EN-3BETA-OL AND 14ALPHA-METHYL-5ALPHA-ERGOSTA-8,24(28)-DIEN-3BETA-OL FROM TRIPARANOL-TREATED CHLORELLA-EMERSONII [J].

DOYLE, PJ ;

PATTERSON, GW ;

DUTKY, SR ;

COHEN, CF .

PHYTOCHEMISTRY, 1971, 10 (09) :2093-+

[8] TRIPARANOL INHIBITION OF STEROL BIOSYNTHESIS IN CHLORELLA-EMERSONII [J].

DOYLE, PJ ;

PATTERSON, GW .

PHYTOCHEMISTRY, 1972, 11 (06) :1951-+

[9]

ELDER JW, 1977, PHYTOCHEMISTRY, V16, P489

[10] 24-METHYLENE LOPHENOL C-28 METHYLTRANSFERASE FROM SUSPENSION CULTURES OF BRAMBLE CELLS [J].

FONTENEAU, P ;

HARTMANNBOUILLON, MA ;

BENVENISTE, P .

PLANT SCIENCE LETTERS, 1977, 10 (02) :147-155

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