SMALL-CELL LUNG-CARCINOMA CELL-LINES EXPRESS MESSENGER-RNA FOR CALCITONIN AND ALPHA-CALCITONIN AND BETA-CALCITONIN GENE-RELATED PEPTIDES

被引：23

作者：

KELLEY, MJ

SNIDER, RH

BECKER, KL

JOHNSON, BE

机构：

[1] VET AFFAIRS MED CTR,WASHINGTON,DC 20422

[2] GEORGE WASHINGTON UNIV,WASHINGTON,DC 20037

来源：

CANCER LETTERS | 1994年 / 81卷 / 01期

关键词：

CALCITONIN; CALCITONIN GENE RELATED PEPTIDE; LUNG NEOPLASM; TUMOR CELL LINES; MESSENGER-RNA; RADIOIMMUNOASSAY; NEUROPEPTIDE;

D O I：

10.1016/0304-3835(94)90159-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Calcitonin (CT) and calcitonin gene related peptide (CGRP) are derived from preprohormones encoded by three mRNAs (CT, alpha-CGRP and beta-CGRP) from two genes (CALC1 and CALC2) on chromosome 11. Among 16 small cell lung cancer cell lines examined by RNase protection assay, 9 (56%) had detectable CT mRNA, 8 (50%) had alpha-CGRP mRNA, and 13 (81%) had beta-CGRP mRNA. At least one CALC1 transcript (CT or alpha-CGRP) was found in 11 (69%) cell lines with three having only CT mRNA, two having only alpha-CGRP mRNA, and six having both. beta-CGRP mRNA was detected in all of these 11 cell lines expressing a CALC1 transcript. Immunoreactive CT was detected by radioimmunoassay in eight of nine SCLC cell lines expressing CT mRNA, and immunoreactive CGRP was detected in 12 of 13 cell lines expressing a CGRP mRNA. The variety of expression of these three peptides in different cell lines of the same cell type should provide a useful system for further study of the control of expression of these peptides.

引用

页码：19 / 25

页数：7

共 23 条

[1] ALTERNATIVE RNA PROCESSING IN CALCITONIN GENE-EXPRESSION GENERATES MESSENGER-RNAS ENCODING DIFFERENT POLYPEPTIDE PRODUCTS [J].