COMPARISON OF THE NUMBER OF IL-4 AND IFN-GAMMA SECRETING CELLS IN RESPONSE TO THE MALARIA VACCINE CANDIDATE ANTIGEN PF155/RESA IN 2 GROUPS OF NATURALLY PRIMED INDIVIDUALS LIVING IN A MALARIA ENDEMIC AREA IN BURKINA-FASO

被引:16
作者
ELGHAZALI, G
ESPOSITO, F
TROYEBLOMBERG, M
机构
[1] UNIV CAMERINO, DEPT BIOL, I-62032 CAMERINO, ITALY
[2] CTR LUTTE CONTRE PALUDISME, OUAGADOUGOU, BURKINA FASO
关键词
D O I
10.1111/j.1365-3083.1995.tb03623.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The enzyme-linked immunospot (ELISPOT) assay was used to enumerate the number of IFN-gamma and IL-4 producing cells after in vitro stimulation with a highly purified recombinant malaria vaccine candidate antigen (r-Pf155/RESA) or synthetic peptides corresponding to its major T-cell epitopes. Two groups of naturally primed individuals living in rural areas of Burkina Faso were studied. The donors comprised one group of healthy (non-parasitemic) mainly adult people and one parasitemic mainly younger people. IL-4 producing cells were detected in response to PHA but no such cells were detected in response to the malarial antigens. The most frequent IFN-gamma responses were seen with r-Pf155/RESA. Thus, after stimulation with this antigen 52% of the donors responded positively in the ELISPOT assay, while only 17% responded to the synthetic peptides, suggesting that the r-Pf155/RESA contained T-cell epitopes not covered by the peptides used in this study. The number of IFN-gamma producing cells in response to the malarial antigens did not differ between the two groups. However, IFN-gamma levels found in sera from the parasitemic individuals were significantly higher than in those from healthy donors. This latter finding and the lack of differences seen in the number of IFN-gamma producing spots in the two groups indicate that IFN-gamma producing cells may have sequestered to other organs in the parasitemic group.
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页码:39 / 45
页数:7
相关论文
共 39 条
[21]  
MOSMANN TR, 1986, J IMMUNOL, V136, P2348
[22]  
Mshana R N, 1991, J Clin Lab Immunol, V34, P131
[23]  
Ockenhouse C F, 1993, Semin Cell Biol, V4, P297, DOI 10.1006/scel.1993.1036
[24]   ALLERGEN-SPECIFIC AND BACTERIAL ANTIGEN-SPECIFIC T-CELL CLONES ESTABLISHED FROM ATOPIC DONORS SHOW A DIFFERENT PROFILE OF CYTOKINE PRODUCTION [J].
PARRONCHI, P ;
MACCHIA, D ;
PICCINNI, MP ;
BISWAS, P ;
SIMONELLI, C ;
MAGGI, E ;
RICCI, M ;
ANSARI, AA ;
ROMAGNANI, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (10) :4538-4542
[25]   ANTIBODIES IN MALARIAL SERA TO PARASITE ANTIGENS IN THE MEMBRANE OF ERYTHROCYTES INFECTED WITH EARLY ASEXUAL STAGES OF PLASMODIUM-FALCIPARUM [J].
PERLMANN, H ;
BERZINS, K ;
WAHLGREN, M ;
CARLSSON, J ;
BJORKMAN, A ;
PATARROYO, ME ;
PERLMANN, P .
JOURNAL OF EXPERIMENTAL MEDICINE, 1984, 159 (06) :1686-1704
[26]   IGE ELEVATION AND IGE ANTIMALARIAL ANTIBODIES IN PLASMODIUM-FALCIPARUM MALARIA - ASSOCIATION OF HIGH IGE LEVELS WITH CEREBRAL MALARIA [J].
PERLMANN, H ;
HELMBY, H ;
HAGSTEDT, M ;
CARLSON, J ;
LARSSON, PH ;
TROYEBLOMBERG, M ;
PERLMANN, P .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 1994, 97 (02) :284-292
[27]   DISSECTION OF THE HUMAN-ANTIBODY RESPONSE TO THE MALARIA ANTIGEN PF155/RESA INTO EPITOPE SPECIFIC COMPONENTS [J].
PERLMANN, H ;
PERLMANN, P ;
BERZINS, K ;
WAHLIN, B ;
TROYEBLOMBERG, M ;
HAGSTEDT, M ;
ANDERSSON, I ;
HOGH, B ;
PETERSEN, E ;
BJORKMAN, A .
IMMUNOLOGICAL REVIEWS, 1989, 112 :115-132
[28]   PURIFICATION, FRACTIONATION AND ASSAY OF ANTIBODY-DEPENDENT LYMPHOCYTIC EFFECTOR CELLS (K-CELLS) IN HUMAN-BLOOD [J].
PERLMANN, H ;
PERLMANN, P ;
PAPE, GR ;
HALLDEN, G .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1976, :57-68
[29]   LYMPHOKINE PRODUCTION BY HUMAN T-CELLS IN DISEASE STATES [J].
ROMAGNANI, S .
ANNUAL REVIEW OF IMMUNOLOGY, 1994, 12 :227-257
[30]  
ROMAGNANI S, 1994, EUR CYTOKINE NETW, V5, P7