BACTERIAL EXPRESSION AND ANALYSIS OF CLEAVAGE ACTIVITY OF HCV SERINE PROTEINASE USING RECOMBINANT AND SYNTHETIC SUBSTRATE

被引：43

作者：

KAKIUCHI, N ^{[1
]}

HIJIKATA, M ^{[1
]}

KOMODA, Y ^{[1
]}

TANJI, Y ^{[1
]}

HIROWATARI, Y ^{[1
]}

SHIMOTOHNO, K ^{[1
]}

机构：

[1] NATL CANC CTR,RES INST,DIV VIROL,CHUO KU,TOKYO 104,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 210卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.1764

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

HCV encoding serine proteinase was expressed in E.coli as a fused form with maltose binding protein(MBP) and a six histidine tag. The enzyme was partially purified by using affinity chromatography for these fused peptides. Proteolytic cleavage activity of the partially purified enzyme was detected by means of an assay using both a recombinant protein and a synthetic peptide substrate which had an amino acid sequence corresponding to the most efficient cleavage site in vivo, the NS5A-NS5B junction. The cleavage occurred at the same site that was reported before. (C) 1995 Academic Press. Inc.

引用

页码：1059 / 1065

页数：7

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