SUPEROXIDE CHEMICAL TRANSFORMATION OF DIOLEPOXIDE POLYAROMATIC HYDROCARBON DNA ADDUCTS - DETERMINATION OF BENZO[A]PYRENE-R-7,T-8,9,C-10-TETRAHYDROTETROL BY GAS-CHROMATOGRAPHY

被引：8

作者：

LI, WN

SOTIRIOULEVENTIS, C

ABDELBAKY, S

FISHER, DH

GIESE, RW

机构：

[1] NORTHEASTERN UNIV,COLL ENGN & APPL SCI,DEPT PHARMACEUT SCI,BOSTON,MA 02115

[2] NORTHEASTERN UNIV,BARNETT INST CHEM ANAL & MAT SCI,BOSTON,MA 02115

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 588卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(91)85033-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Benzo[a]pyrene-r-7,t-8,9,c-10-tetrahydrotetrol (100 pg, 342 fmol) was measured using the following sequence of steps: (1) chemical transformation with potassium superoxide to 2,3-pyrenedicarboxylic acid; (2) electrophore derivatization with pentafluorobenzyl bromide; (3) sample clean-up by high-performance liquid chromatography and (4) measurement by gas chromatography with electron-capture detection and by gas chromatography with electron-capture negative-ion mass spectrometry. The overall, absolute yields obtained by the two procedures were 69% and 60%, respectively. This work completes the first stage towards the establishment of a general method for detecting diolepoxide polyaromatic hydrocarbon DNA adducts by gas chromatography.

引用

页码：273 / 280

页数：8

共 9 条

[1]

BACHMANN WE, 1941, J AM CHEM SOC, V63, P1682

[2]

BAKTHAVACHALAM J, 1990, J CHROMATOGR, V538, P447

[3]

BARTSCH H, 1988, IARC SCI PUBLICATION, V89

[4] DETERMINATION OF 5-METHYLCYTOSINE IN DNA BY GAS-CHROMATOGRAPHY ELECTRON-CAPTURE DETECTION [J].