DODECYL SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS AT LOW PH VALUES AND LOW-TEMPERATURES

被引：42

作者：

LICHTNER, R

WOLF, HU

机构：

来源：

BIOCHEMICAL JOURNAL | 1979年 / 181卷 / 03期

关键词：

D O I：

10.1042/bj1810759

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method is described for dodecyl sulphate/polyacrylamide-gel electrophoresis of pH- and temperature-labile biological intermediates. The method is based on a catalyst system that works at temperatures of 2--4 degrees C and pH values of 2--4 and an appropriate buffer system containing Li+ or Tris [CH2OH--C(CH2OH)2--NH3+] instead of Na+. This system does not lead to the precipitation of 1% dodecyl sulphate.

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页码：759 / 761

页数：3

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