RADIORESISTANT DERIVATIVES OF AN X-RAY-SENSITIVE CHO CELL-LINE EXHIBIT DISTINCT PATTERNS OF SENSITIVITY TO DNA-DAMAGING AGENTS

被引：15

作者：

JAFFE, DR ^{[1
]}

HARAF, D ^{[1
]}

SCHWARTZ, JL ^{[1
]}

WEICHSELBAUM, RR ^{[1
]}

DIAMOND, AM ^{[1
]}

机构：

[1] UNIV CHICAGO,MED CTR,DEPT RADIAT & CELLULAR ONCOL,5842 S MARYLAND AVE,CHICAGO,IL 60637

来源：

CARCINOGENESIS | 1990年 / 11卷 / 08期

关键词：

D O I：

10.1093/carcin/11.8.1265

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

X-ray-resistant clones of the xrs-5 radiosensitive derivative of the CHO-K1 cell line were generated by transfecting cosmid library DNAs into the X-ray-sensitive cells. Transfectants were selected for both a dominant drug resistance marker present in the vector sequences and return to wild-type survival. Three cell lines were isolated which show X-ray survival characteristics similar to parental K1 cells. These revertant lines were examined for their cross-sensitivity to cis-diamminedichloroplatinum(II) (cisplatin) and bleomycin. Although these cell lines reverted with regard to X-ray sensitivity, they retained their sensitivity to cisplatin. Furthermore, changes in bleomycin and X-ray sensitivity did not correlate. There was a positive correlation between return to wild-type radiosensitivity and an increase In the rate of DNA double-strand break rejoining. © 1990 Oxford University Press.

引用

页码：1265 / 1269

页数：5