DETECTION OF BORNA-DISEASE VIRUS-RNA IN FORMALIN-FIXED, PARAFFIN-EMBEDDED BRAIN-TISSUES BY NESTED PCR

被引：32

作者：

SORG, I

METZLER, A

机构：

[1] UNIV ZURICH,FAC VET MED,INST VET BACTERIOL,CH-8057 ZURICH,SWITZERLAND

[2] UNIV ZURICH,FAC VET MED,INST VIROL,CH-8057 ZURICH,SWITZERLAND

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 04期

关键词：

D O I：

10.1128/JCM.33.4.821-823.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A method for detecting Borna disease virus (BDV) RNA in formalin-fixed, paraffin-embedded brain tissue sections was established. By digestion with proteinase K and subsequent extraction with guanidinium thiocyanate, phenol, and chloroform, we were able to efficiently release RNA from the fixed tissues. By reverse transcription of the RNA and nested PCR a 212-bp product was generated, as expected.

引用

页码：821 / 823

页数：3