SIMPLE METHODS FOR PURIFICATION OF CRUDE K-CASEIN AND BETA-CASEIN BY TREATMENT WITH CALCIUM PHOSPHATE GEL

Batch methods applicable on a large scale are described for the purification of crude κ- and β κ-Casein, dissolved in urea-containing buffer, was freed from αs- and β-caseins by treatment with calcium phosphate gel and recovered in about 60% yield. β-Casein was freed from most impurities by adsorption on to calcium phosphate gel at pH 7.8 in the presence of urea and elution with 6 M-urea–N-NH4OH at 4°C. The recovery was about 50%. © 1969, Proprietors of Journal of Dairy Research. All rights reserved.