HIGH-RESOLUTION SCANNING ELECTRON-MICROSCOPY OF ISOLATED AND INSITU CYTOSKELETAL ELEMENTS

被引：58

作者：

IP, W

FISCHMAN, DA

机构：

来源：

JOURNAL OF CELL BIOLOGY | 1979年 / 83卷 / 01期

关键词：

D O I：

10.1083/jcb.83.1.249

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Evidence is presented that cytoskeletal (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different classes of filaments or tubules can be identified both as purified protein polymers and as structured organelles within cryofractured or detergent-extracted cells.

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页码：249 / 254

页数：6

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