DIAZEPAM ENHANCES INTRASYNAPTOSOMAL FREE CALCIUM CONCENTRATIONS

被引：7

作者：

MARTIN, JV

KEIR, DR

LEE, H

机构：

[1] Department of Biology, Rutgers University, Camden

来源：

BRAIN RESEARCH | 1991年 / 548卷 / 1-2期

关键词：

BENZODIAZEPINE; CALCIUM ION; DIAZEPAM; FURA-2; FURA-2/AM; SYNAPTOSOME;

D O I：

10.1016/0006-8993(91)91125-K

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Synaptosomes prepared from brains of rats were incubated in different concentrations of diazepam under conditions designed to reduce the action of a reversed Na+/Ca2+ exchanger. In synaptosomes depolarized in the presence of added Ca2+, doses of diazepam ranging from 0.1 to 100-mu-M were found to significantly enhance Ca2+ levels measured with the fluorescent dye fura-2, compared to control incubations without drug. Furthermore, doses of diazepam as low as 1-mu-M significantly increased the concentration of Ca2+ in non-depolarized synaptosomes without added Ca2+ in the medium. The effects of depolarization and diazepam treatment were synergistic increasing the levels of intrasynaptosomal Ca2+.

引用

页码：222 / 227

页数：6

共 41 条

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