THE EFFECTS OF PH, IONIC-STRENGTH AND POLYVALENT IONS ON THE CELL-SURFACE HYDROPHOBICITY OF ESCHERICHIA-COLI EVALUATED BY THE BATH AND HIC METHODS

被引：28

作者：

BUNT, CR

JONES, DS

TUCKER, IG

机构：

[1] School of Pharmacy, University of Otago, Dunedin

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 1995年 / 113卷 / 02期

关键词：

ADHERENCE; BATH; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; PH; TONIC STRENGTH; POLYVALENT ION; ESCHERICHIA COLI;

D O I：

10.1016/0378-5173(94)00205-J

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effects of pH, ionic strength and polyvalent ions upon the subsequent adherence of E. coli to octyl-sepharose in hydrophobic interaction chromatography (HIC), and the effect of polyvalent ions on adherence to dichloromethane in the bacterial adherence to hydrocarbons (BATH) tests were investigated. Ionic strength, pH and polyvalent ions were found to influence adhesion. Lowering pH and increasing ionic strength increased adherence of E. coli to octyl-Sepharose and dichloromethane. However, polyvalent ions increased adherence to octyl-Sepharose while reducing adherence to dichloromethane, indicating the possibility of different mechanisms of interaction between E. coli and these substrates.

引用

页码：257 / 261

页数：5

共 33 条

[1]

ABSOLOM DR, 1982, T AM SOC ART INT ORG, V28, P413

[2]

ARAKAWA T, 1991, BIOTECHNOL APPL BIOC, V13, P151

[3] INFLUENCE OF THE GROWTH-CONDITIONS ON THE HYDROPHOBICITY OF RENIBACTERIUM-SALMONINARUM EVALUATED BY DIFFERENT METHODS [J].