POLYMERASE CHAIN-REACTION AMPLIFICATION OF RABIES VIRUS NUCLEIC-ACIDS FROM TOTAL MOUSE-BRAIN RNA

被引：13

作者：

ERMINE, A ^{[1
]}

LARZUL, D ^{[1
]}

CECCALDI, PE ^{[1
]}

GUESDON, JL ^{[1
]}

TSIANG, H ^{[1
]}

机构：

[1] INST PASTEUR,SONDES FROIDES LAB,F-75724 PARIS 15,FRANCE

来源：

MOLECULAR AND CELLULAR PROBES | 1990年 / 4卷 / 03期

关键词：

dot-blot hybridization; polymerase chain reaction; rabies virus; RNA genome;

D O I：

10.1016/0890-8508(90)90052-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In an attempt to improve the sensitivity of the rabies genome hybridization test, PCR amplification was used following reverse transcription of rabies RNA extracted from infected brain. Presence of amplified DNA is demonstrated with either cDNA synthesized from the antigenomic primer or from antimessenger primer. © 1990.

引用

页码：189 / 191

页数：3

共 8 条

[1] RAPID DIAGNOSIS OF RABIES INFECTION BY MEANS OF A DOT HYBRIDIZATION ASSAY
ERMINE, A
TORDO, N
TSIANG, H
[J]. MOLECULAR AND CELLULAR PROBES, 1988, 2 (01) : 75 - 82
[2] ERMINE A, 1989, RAPID METHODS AUTOMA, P513
[3] A NON-RADIOACTIVE DIAGNOSTIC-TEST FOR THE DETECTION OF HBV DNA-SEQUENCES IN SERUM AT THE SINGLE MOLECULE LEVEL
LARZUL, D
CHEVRIER, D
GUESDON, JL
[J]. MOLECULAR AND CELLULAR PROBES, 1989, 3 (01) : 45 - 57
[4] DETECTION OF HEPATITIS-B VIRUS SEQUENCES IN SERUM BY USING INVITRO ENZYMATIC AMPLIFICATION
LARZUL, D
GUIGUE, F
SNINSKY, JJ
MACK, DH
BRECHOT, C
GUESDON, JL
[J]. JOURNAL OF VIROLOGICAL METHODS, 1988, 20 (03) : 227 - 237
[5] SEQUENCE OF THE 3386-3' NUCLEOTIDES OF THE GENOME OF THE AVO1 STRAIN RABIES VIRUS - STRUCTURAL SIMILARITIES IN THE PROTEIN REGIONS INVOLVED IN TRANSCRIPTION
POCH, O
TORDO, N
KEITH, G
[J]. BIOCHIMIE, 1988, 70 (08) : 1019 - 1029
[6] ENZYMATIC AMPLIFICATION OF BETA-GLOBIN GENOMIC SEQUENCES AND RESTRICTION SITE ANALYSIS FOR DIAGNOSIS OF SICKLE-CELL ANEMIA
SAIKI, RK
SCHARF, S
FALOONA, F
MULLIS, KB
HORN, GT
ERLICH, HA
ARNHEIM, N
[J]. SCIENCE, 1985, 230 (4732) : 1350 - 1354
[7] PRIMARY STRUCTURE OF LEADER RNA AND NUCLEOPROTEIN GENES OF THE RABIES GENOME - SEGMENTED HOMOLOGY WITH VSV
TORDO, N
POCH, O
ERMINE, A
KEITH, G
[J]. NUCLEIC ACIDS RESEARCH, 1986, 14 (06) : 2671 - 2683
[8] Webster W.A., 1988, P201

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