THE GENOMIC REGION ENCODING TOXIN-GAMMA FROM THE SCORPION TITYUS-SERRULATUS CONTAINS AN INTRON

被引：32

作者：

BECERRIL, B

CORONA, M

MEJIA, MC

MARTIN, BM

LUCAS, S

BOLIVAR, F

POSSANI, LD

机构：

[1] UNIV NACL AUTONOMA MEXICO, INST BIOTECNOL, CUERNAVACA 62271, MORELOS, MEXICO

[2] NIMH, MOLEC NEUROGENET UNIT, CLIN NEUROSCI BRANCH, BETHESDA, MD 20892 USA

[3] INST BUTANTAN, SAO PAULO, BRAZIL

来源：

FEBS LETTERS | 1993年 / 335卷 / 01期

关键词：

SCORPION TOXIN; NA+ CHANNEL; GENOMIC DNA CLONE; NUCLEOTIDE SEQUENCE; ARACHNID INTRON; TITYUS SERRULATUS;

D O I：

10.1016/0014-5793(93)80428-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene encoding toxin gamma from the scorpion, Tityus serrulatus, was amplified by PCR from genomic DNA employing synthetic oligonucleotides designed from the reported cDNA sequence. The nucleotide sequence of this gene reveals the presence of an intron of 475 base pairs (bp) which interrupts the region that encodes the signal peptide of the precursor toxin. A comparison of the intron boundary sequences of the gamma toxin gene with ones from other arachnid genes is also presented.

引用

页码：6 / 8

页数：3

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