CHLORIDE AND MONOVALENT ION-SELECTIVE CATION CURRENTS ACTIVATED BY OXYTOCIN IN PREGNANT RAT MYOMETRIAL CELLS

被引：31

作者：

ARNAUDEAU, S ^{[1
]}

LEPRETRE, N ^{[1
]}

MIRONNEAU, J ^{[1
]}

机构：

[1] UNIV BORDEAUX 2,PHYSIOL CELLULAIRE & PHARMACOL MOLEC LAB,CNRS,URA 1489,F-33076 BORDEAUX,FRANCE

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 1994年 / 171卷 / 02期

关键词：

CHLORIDE CURRENT; CATION CURRENT; FREE CYTOSOLIC CALCIUM; OXYTOCIN; MYOMETRIUM;

D O I：

10.1016/0002-9378(94)90288-7

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

OBJECTIVE: The purpose of our study was to characterize the membrane mechanisms responsible for oxytocin-induced depolarization in single cells from pregnant rat myometrium. STUDY DESIGN: Membrane currents were recorded with the whole-cell mode of the standard patch-clamp technique. Intracellular calcium concentration was monitored with the fluorescence from Fura 2 added to the pipette solution. RESULTS: We found that oxytocin predominantly activates potassium, chloride, and cation conductances. Chloride and cation currents were evoked by an increase in the intracellular calcium concentration dependent on calcium release from the heparin-sensitive intracellular stores. Chloride and cation current showed different calcium dependences so that they could be activated separately. CONCLUSION: Stimulation of oxytocin receptors induces opening of calcium-activated chloride and cation channels, leading to depolarization of the myometrial cells. This depolarization opens, in turn, voltage-dependent calcium channels.

引用

页码：491 / 501

页数：11

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