EVALUATION OF MIXED-MODE STATIONARY PHASES IN LIQUID-CHROMATOGRAPHY FOR THE SEPARATION OF CHARGED AND UNCHARGED OLIGOMER-LIKE MODEL COMPOUNDS

被引:26
作者
ELEVELD, JT
CLAESSENS, HA
AMMERDORFFER, JL
VANHERK, AM
CRAMERS, CA
机构
[1] EINDHOVEN UNIV TECHNOL,INSTRUMENTAL ANAL LAB,5600 MB EINDHOVEN,NETHERLANDS
[2] EINDHOVEN UNIV TECHNOL,POLYMER CHEM LAB,5600 MB EINDHOVEN,NETHERLANDS
关键词
D O I
10.1016/0021-9673(94)80150-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The possibility to separate charged and uncharged oligomer-like test compounds by high-performance liquid chromatography was investigated, using mixed-mode (reversed-phase/anion-exchange) stationary phases. In order to examine this possibility, several columns were evaluated for their reversed-phase, anion-exchange and mixed-mode properties using neutral and charged model compounds. The OmniPac PAX-500, the PRP-X100 and the mixed-phase PLRP-S/PL-SAX columns showed promising results for the separation of real samples.
引用
收藏
页码:211 / 227
页数:17
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