2,3,5-TRIPHENYLTETRAZOLIUM CHLORIDE AS A VIABILITY ASSAY FOR IMMOBILIZED PLANT CELLS

被引：72

作者：

Iborra, J. L. ^{[1
]}

Guardiola, J. ^{[1
]}

Montaner, S. ^{[1
]}

Canovas, M. ^{[1
]}

Manjon, A. ^{[1
]}

机构：

[1] Univ Murcia, Fac Chem, Dept Biochem & Mol Biol B & Immunol, E-30001 Murcia, Spain

来源：

BIOTECHNOLOGY TECHNIQUES | 1992年 / 6卷 / 04期

关键词：

D O I：

10.1007/BF02439319

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Grape (V. vinifera L. cv Gamay Freaux) cells were entrapped in calcium alginate beads. The effect of bead diameter and gel concentration on the viability of the cells was checked. The reduction of 2,3,5-triphenyltetrazolium chloride as well as O-2 consumption were used as viability test, and their results compared. The existence of diffusional limitations for O-2 introduces an unaccuracy as high as 25% for the O-2 consumption method. The reduction assay is more simple, precise and reliable.

引用

页码：319 / 322

页数：4

共 9 条

[1]

METHODS FOR THE ESTIMATION OF THE NUMBER AND QUALITY OF ANIMAL-CELLS IMMOBILIZED IN CARBOHYDRATE GELS [J].