D-L AMINO-ACID-ANALYSIS USING AUTOMATED PRECOLUMN DERIVATIZATION WITH 1-FLUORO-2,4-DINITROPHENYL-5-ALANINE AMIDE

被引：14

作者：

SCALONI, A

SIMMACO, M

BOSSA, F

机构：

[1] Dipartimento di Scienze Biochimiche 'A. Rossi Fanelli', Centro di Biologia Molecolare del Consiglio Nazionale delle Ricerche, Università 'La Sapienza', Roma, I-00185

来源：

AMINO ACIDS | 1995年 / 8卷 / 03期

关键词：

AMINO ACID ENANTIOMERIC MIXTURES; MARFEY REAGENT; EDMAN DEGRADATION; RP-HPLC;

D O I：

10.1007/BF00806826

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An automated procedure for the precolumn derivatization of enantiomeric amino acid mixtures with 1-fluoro-2,4-dinitrophenyl-5-alanine amide and a liquid chromatographic method for the separation of the derivatives with UV detection are reported. The system described allows to perform routine analyses using microbore columns with a sensitivity at the picomol level. Improvements for the use of this reagent in the protocol of a subtractive Edman degradation procedure of peptides to determine the sequence position of amino acid residues with concomitant identification of their chirality are also described.

引用

页码：305 / 313

页数：9

共 21 条

[1]

Bachmann RC., 1994, NAT PROD LETT, V4, P159, DOI 10.1080/10575639408043899

[2]

BADA JL, 1984, METHOD ENZYMOL, V106, P98

[3] AUTOMATED ENANTIOSEPARATION OF AMINO-ACIDS BY DERIVATIZATION WITH ORTHO-PHTHALDIALDEHYDE AND N-ACYLATED CYSTEINES [J].

BRUCKNER, H ;

WITTNER, R ;

GODEL, H .

JOURNAL OF CHROMATOGRAPHY, 1989, 476 :73-82

[4] LIGAND-EXCHANGE CHROMATOGRAPHY OF RACEMATES INFLUENCE OF DEGREE OF SATURATION OF ASYMMETRIC RESIN BY METAL-IONS ON LIGAND-EXCHANGE [J].

DAVANKOV, VA ;

ROGOZHIN, SV ;

SEMECHKI.AV ;

SACHKOVA, TP .

JOURNAL OF CHROMATOGRAPHY, 1973, 82 (02) :359-365

[5] SEPARATION OF AMINO-ACID ENANTIOMERS AND CHIRAL AMINES USING PRECOLUMN DERIVATIZATION WITH (+)-1-(9-FLUORENYL)ETHYL CHLOROFORMATE AND REVERSED-PHASE LIQUID-CHROMATOGRAPHY [J].

EINARSSON, S ;

JOSEFSSON, B ;

MOLLER, P ;

SANCHEZ, D .

ANALYTICAL CHEMISTRY, 1987, 59 (08) :1191-1195

[6] RESOLUTION OF THE OPTICAL ISOMERS OF UNDERIVATIZIZED AMINO-ACIDS ON CHEMICALLY BONDED CHIRAL PHASES BY LIGAND-EXCHANGE CHROMATOGRAPHY [J].

GUBITZ, G ;

JELLENZ, W ;

SANTI, W .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1981, 4 (04) :701-712

[7] SEPARATION OF D-AMINO AND L-AMINO-ACIDS BY LIQUID-CHROMATOGRAPHY - USE OF CHIRAL ELUANTS [J].

HARE, PE ;

GILAV, E .

SCIENCE, 1979, 204 (4398) :1226-1228

[8] DETERMINATION OF FREE D-AMINO ACIDS WITH A BACTERIAL TRANSAMINASE - THEIR DEPLETION LEADS TO INHIBITION OF BACTERIAL-GROWTH [J].

JONES, WM ;

RINGE, D ;

SODA, K ;

MANNING, JM .

ANALYTICAL BIOCHEMISTRY, 1994, 218 (01) :204-209

[9]

KOCHHAR S, 1989, ANAL BIOCHEM, V1178, P17

[10] RACEMIZATION RATES OF AMINO-ACIDS FOR DATING ANCIENT SAMPLES [J].

LUBEC, G ;

LUBEC, B .

AMINO ACIDS, 1993, 4 (1-2) :1-3

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