ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR MEASLES ANTIBODY - A COMPARISON WITH HEMAGGLUTINATION INHIBITION, IMMUNOFLUORESCENCE AND PLAQUE NEUTRALIZATION TESTS

被引：16

作者：

DESOUZA, VAUF ^{[1
]}

PANNUTI, CS ^{[1
]}

SUMITA, LM ^{[1
]}

ALBRECHT, P ^{[1
]}

机构：

[1] US FDA, DIV VIROL, BETHESDA, MD 20014 USA

来源：

REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO | 1991年 / 33卷 / 01期

关键词：

MEASLES; ELISA; PLAQUE NEUTRALIZATION; IMMUNOFLUORESCENCE; HEMAGGLUTINATION INHIBITION;

D O I：

10.1590/S0036-46651991000100007

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

An enzyme-linked immunosorbent assay (ELISA) for measles antibodies was compared with Plaque Neutralization (PRN), Haemagglutination inhibition (HI) and Fluorescent antibody (IFA) tests in 181 sera from vaccinated children and umbilical cord. Of 179 positive samples by the sensitive PRN, only two, with titers of 8, were negative by ELISA (copositivity of 98.9%). IFA and HI presented, respectively, copositivities of 93.3% and 82.7%. The ELISA presented a high sensitivity as well as a good reproducibility and represents an alternative for the time consuming PRN for detection of low measles antibodies.

引用

页码：32 / 36

页数：5

共 13 条

[1] ROLE OF VIRUS-STRAIN IN CONVENTIONAL AND ENHANCED MEASLES PLAQUE NEUTRALIZATION TEST [J].