ANALYSIS OF CHONDROITIN SULFATE ISOMERS IN THE PERIODONTIUM OF THE MONKEY USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：18

作者：

OKAZAKI, J ^{[1
]}

KAMADA, A ^{[1
]}

GONDA, Y ^{[1
]}

SAKAKI, T ^{[1
]}

机构：

[1] OSAKA DENT UNIV,DEPT BIOCHEM,OSAKA 540,JAPAN

来源：

JOURNAL OF PERIODONTAL RESEARCH | 1992年 / 27卷 / 05期

关键词：

CHONDROITIN SULFATE; UNSATURATED DISACCHARIDE; PERIODONTIUM; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;

D O I：

10.1111/j.1600-0765.1992.tb01821.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Glycosaminoglycan (GAG) was extracted from monkey periodontium, consisting of gingiva, periodontal ligament, alveolar bone and cementum, and from dental pulp and dentin by digestion with Pronase E. Unsaturated disaccharide isomers formed by chondroitinase AC digestion from chondroitin sulfate were labeled with dansylhydrazine and analyzed by high-performance liquid chromatography. These tissues showed different molar ratios of the unsaturated chondroitin sulfate disaccharides. The ratio of DELTADi-4S to DELTADi-6S was lowest in the dental pulp, followed by the gingiva, periodontal ligament, dentin alveolar bone, and cementum, in that order. It was greater in the calcified than in the uncalcified tissues.

引用

页码：484 / 488

页数：5

共 18 条

[1] GLYCOSAMINOGLYCANS OF HUMAN CEMENTUM [J].