CLONING AND CHARACTERIZATION OF A BOVINE ALPHA-INTERFERON RECEPTOR

被引：26

作者：

LIM, JK ^{[1
]}

LANGER, JA ^{[1
]}

机构：

[1] UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT MOLEC GENET & MICROBIOL,PISCATAWAY,NJ 08854

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1173卷 / 03期

关键词：

INTERFERON RECEPTOR; ALPHA-INTERFERON; PROTEIN CROSS-LINKING; CDNA SEQUENCE;

D O I：

10.1016/0167-4781(93)90129-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A bovine interferon a receptor (BoIFN-alphaR1) cDNA, homologous to the human cDNA [1], was isolated. Transfection of the BoIFN-alphaR1 cDNA into monkey COS cells results in a large increase in high-affinity binding sites for human IFN-alphaA and IFN-alphaB. Covalent crosslinking of radiolabeled HuIFN-alphaA and -alphaB demonstrates that the complex of [P-32]HuIFN with the BoIFN-alphaR1 protein (predicted mass, 61375) expressed in COS cells migrates as a 140-150 kDa band.

引用

页码：314 / 319

页数：6

共 41 条

[1] STRUCTURAL DESIGN AND MOLECULAR EVOLUTION OF A CYTOKINE RECEPTOR SUPERFAMILY [J].