学术探索
学术期刊
新闻热点
数据分析
智能评审

RAT (AND MOUSE) MONOCLONAL-ANTIBODIES .8. ELISA MEASUREMENT OF IG PRODUCTION IN MOUSE HYBRIDOMA CULTURE SUPERNATANTS

被引：12
作者：
DELAUNAY, T
LOUAHED, J
BAZIN, H
机构：
[1] UNIV CATHOLIQUE LOUVAIN,EXPTL IMMUNOL UNIT,CLOS CHAPELLE CHAMPS 3056,B-1200 BRUSSELS,BELGIUM
[2] HOECHST BEHRING,RECH & NOUVEAUX DEV,RUEIL MALMAISON,FRANCE
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 131卷 / 01期
关键词：
ELISA; Immunoglobulin; mouse; Monoclonal antibody; rat; Protein determination;
D O I：
10.1016/0022-1759(90)90229-O
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a rat monoclonal antibody (MAb)-based solid-phase immunoenzymometric assay for the quantification of mouse hybridoma culture supernatant immunoglobulins (Igs). This assay involves the use of two rat MAbs, LO-MK-1 and LO-MK-2, which bind distinct mouse κ light chain epitopes expressed by all murine κ Igs. The assay permits reliable measurement of all murine κ IgG subclasses in the 2-120 ng/ml range and murine κ IgM class in the 2-30 ng/ml range. The intra- and interassay coefficients of variation in the measurement of Ig in mouse hybridoma culture supernatants averaged 7.5% and 5% respectively. The assay is simple, reproducible, rapid and does not require specific equipment. It is of potential value to all laboratories engaged in hybridoma technology. © 1990.
引用
收藏
页码：33 / 39
页数：7
相关论文
共 17 条
[1]  
BAZIN H, 1990, RAT HYBRIDOMAS RAT M, P515
[2]  
BAZIN H, 1982, PROTIDES BIOL FLUIDS, P615
[3]   ELISA MEASUREMENT OF IGG SUBCLASS PRODUCTION IN CULTURE SUPERNATANTS USING MONOCLONAL-ANTIBODIES [J].
BIRD, P ;
CALVERT, JE ;
LOWE, J ;
DUGGANKEEN, M ;
FOROUHI, NG ;
SEPPALA, I ;
LING, NR .
JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 104 (1-2) :149-158
[4]   THE IMMUNOCHEMISTRY OF SANDWICH ELISAS .1. THE BINDING CHARACTERISTICS OF IMMUNOGLOBULINS TO MONOCLONAL AND POLYCLONAL CAPTURE ANTIBODIES ADSORBED ON PLASTIC AND THEIR DETECTION BY SYMMETRICAL AND ASYMMETRICAL ANTIBODY-ENZYME CONJUGATES [J].
BUTLER, JE ;
SPRADLING, JE ;
SUTER, M ;
DIERKS, SE ;
HEYERMANN, H ;
PETERMAN, JH .
MOLECULAR IMMUNOLOGY, 1986, 23 (09) :971-982
[5]   ALTERED RECOGNITION OF SURFACE-ADSORBED COMPARED TO ANTIGEN-BOUND ANTIBODIES IN THE ELISA [J].
DIERKS, SE ;
BUTLER, JE ;
RICHERSON, HB .
MOLECULAR IMMUNOLOGY, 1986, 23 (04) :403-411
[6]   MEASUREMENT OF THE CONCENTRATION OF MURINE IGG MONOCLONAL-ANTIBODY IN HYBRIDOMA SUPERNATANTS AND ASCITES IN ABSOLUTE UNITS BY SENSITIVE AND RELIABLE ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISA) [J].
FLEMING, JO ;
PEN, LB .
JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 110 (01) :11-18
[7]   MEASUREMENTS OF THE TRUE AFFINITY CONSTANT IN SOLUTION OF ANTIGEN-ANTIBODY COMPLEXES BY ENZYME-LINKED IMMUNOSORBENT-ASSAY [J].
FRIGUET, B ;
CHAFFOTTE, AF ;
DJAVADIOHANIANCE, L ;
GOLDBERG, ME .
JOURNAL OF IMMUNOLOGICAL METHODS, 1985, 77 (02) :305-319
[8]  
HOWANITZ PJ, 1988, ARCH PATHOL LAB MED, V112, P771
[9]   USE OF MONOCLONAL-ANTIBODIES TO QUANTIFY SUBCLASSES OF HUMAN-IGG .1. DEVELOPMENT OF 2-SITE IMMUNOENZYMOMETRIC ASSAYS FOR TOTAL IGG SUBCLASS DETERMINATIONS [J].
HUSSAIN, R ;
POINDEXTER, RW ;
WISTAR, R ;
REIMER, CB .
JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 93 (01) :89-96
[10]   INVITRO PRODUCTION OF MONOCLONAL-ANTIBODIES UNDER SERUM-FREE CONDITIONS USING A COMPACT AND INEXPENSIVE HOLLOW FIBER CELL-CULTURE UNIT [J].
KLERX, JPAM ;
VERPLANKE, CJ ;
BLONK, CG ;
TWAALFHOVEN, LC .
JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 111 (02) :179-188
12