THE USE OF SALT HYDRATES AS WATER BUFFERS TO CONTROL ENZYME-ACTIVITY IN ORGANIC-SOLVENTS

被引：15

作者：

ROBB, DA

YANG, Z

HALLING, PJ

机构：

[1] Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow

来源：

BIOCATALYSIS | 1994年 / 9卷 / 1-4期

关键词：

TYROSINASE (EC 1.10.14.1); BIOCATALYSIS IN ORGANIC SOLVENTS; WATER ACTIVITY;

D O I：

10.3109/10242429408992127

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conversion of o-diphenols to o-quinones was carried out in chloroform using a dry powder prepared from mushrooms as the catalyst. Several salt hydrates proved effective in supplying the small amount of water necessary for catalysis. The efficacy of the hydrates was related to their water activities and their use provided a convenient method for controlling water activity in nearly non-aqueous conditions.

引用

页码：277 / 283

页数：7

共 13 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

FLING M, 1963, J BIOL CHEM, V238, P2045

[3] ORGANIC LIQUIDS AND BIOCATALYSTS - THEORY AND PRACTICE [J].