SALT HYDRATES BUFFER WATER ACTIVITY DURING CHYMOTRYPSIN-CATALYZED PEPTIDE-SYNTHESIS

被引：50

作者：

KUHL, P

HALLING, PJ

机构：

[1] UNIV STRATHCLYDE,DEPT BIOSCI & BIOTECHNOL,GLASGOW G1 1XW,SCOTLAND

[2] KARL MARX UNIV,SEKT BIOWISSENSCH,O-7010 LEIPZIG,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1078卷 / 03期

关键词：

ORGANIC MEDIA; SALT HYDRATE; WATER ACTIVITY; PEPTIDE SYNTHESIS;

D O I：

10.1016/0167-4838(91)90151-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chymotrypsin (EC 3.4.21.1) powder suspended in hexane in the presence of Na2CO3.10H2O is a good catalyst for peptide synthesis. The salt hydrate releases water to fix the thermodynamic water activity of the system in accord with its dissociation pressure. Salt hydrates can be useful to buffer water activity in mainly organic enzyme reaction mixtures at a value permitting activity of the catalyst while minimising hydrolytic side reactions.

引用

页码：326 / 328

页数：3

共 11 条

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