MOLECULAR-CLONING AND EXPRESSION OF A DELTA-OPIOID RECEPTOR FROM RAT-BRAIN

被引：46

作者：

ABOOD, ME

NOEL, MA

FARNSWORTH, JS

TAO, Q

机构：

[1] Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonweath University, Richmond

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 1994年 / 37卷 / 06期

关键词：

RAT; CDNA-BRAIN; DOR-1;

D O I：

10.1002/jnr.490370605

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have isolated and characterized a rat delta-opioid receptor. The deduced amino acid sequence (372 aa) closely resembles the murine delta-opioid receptor, DOR-1. In fact, 97% of the amino acid residues are conserved between the two species, while 93% of the nucleic acid residues are identical. A 6 kb mRNA was detected in rat cortex using rat DOR-1 as a probe. When expressed in COS cells, the clone shows high affinity opioid binding with selectivity for delta-opioids. The rat delta-opioid receptor cDNA clone will be a useful tool for studying the function of delta-opioid receptor in rats. (C) 1994 Wiley-Liss, Inc.

引用

页码：714 / 719

页数：6

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