HIGH-MOLECULAR-WEIGHT PROTEASE COMPLEXES (PROTEASOMES) OF SPERM OF THE ASCIDIAN, HALOCYNTHIA-RORETZI - ISOLATION, CHARACTERIZATION, AND PHYSIOLOGICAL ROLES IN FERTILIZATION

被引:50
作者
SAITOH, Y [1 ]
SAWADA, H [1 ]
YOKOSAWA, H [1 ]
机构
[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,DEPT BIOCHEM,SAPPORO,HOKKAIDO 060,JAPAN
关键词
D O I
10.1006/dbio.1993.1182
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
It has been proposed that a chymotrypsin-like enzyme plays a key role in sperm penetration through the vitelline coat of eggs of the ascidian, Halocynthia roretzi. In this report, two kinds of chymotrypsin-like enzymes were isolated from spermatozoa of H. roretzi. Both enzymes were characterized as high-molecular-weight protease complexes that comprised several different subunits and that showed several different protease activities. Both showed immunoreactivity with anti-proteasome antibody. One showed properties characteristic of the 20 S proteasome and its molecular mass was 630 kDa, whereas the other protease complex of 930 kDa showed inhibitor susceptibility different from that of the 20 S proteasome. Upon the sperm reaction which is induced by treatment with high pH, the proteasomes were found to be released from sperm and they had the activity to digest the vitelline coats. These results suggest that the proteasomes are exposed extracellularly when sperm contact with the egg coat and may function as lysins in the fertilization process. © 1993 by Academic Press, Inc.
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页码:238 / 244
页数:7
相关论文
共 30 条
[21]   TIMING OF ACTION OF SPERM PROTEASES IN ASCIDIAN FERTILIZATION [J].
SAWADA, H ;
YOKOSAWA, H ;
NUMAKUNAI, T ;
ISHII, S .
EXPERIENTIA, 1986, 42 (01) :74-75
[22]   ASCIDIAN SPERM CHYMOTRYPSIN-LIKE ENZYME - PARTICIPATION IN FERTILIZATION [J].
SAWADA, H ;
YOKOSAWA, H ;
HOSHI, M ;
ISHII, S .
EXPERIENTIA, 1983, 39 (04) :377-378
[23]  
SAWADA H, 1984, J BIOL CHEM, V259, P2900
[24]   EVIDENCE FOR ACROSIN-LIKE ENZYME IN SPERM EXTRACT AND ITS INVOLVEMENT IN FERTILIZATION OF THE ASCIDIAN, HALOCYNTHIA RORETZI [J].
SAWADA, H ;
YOKOSAWA, H ;
HOSHI, M ;
ISHII, S .
GAMETE RESEARCH, 1982, 5 (03) :291-301
[25]   EVIDENCE FOR THE PARTICIPATION OF 2 SPERM PROTEASES, SPERMOSIN AND ACROSIN, IN FERTILIZATION OF THE ASCIDIAN, HALOCYNTHIA-RORETZI - INHIBITORY EFFECTS OF LEUPEPTIN ANALOGS ON ENZYME-ACTIVITIES AND FERTILIZATION [J].
SAWADA, H ;
YOKOSAWA, H ;
SOMENO, T ;
SAINO, T ;
ISHII, S .
DEVELOPMENTAL BIOLOGY, 1984, 105 (01) :246-249
[26]  
TANAKA K, 1992, NEW BIOL, V4, P173
[27]  
TILNEY LG, 1985, BIOL FERTILIZATION, V2, P157
[28]   ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].
TOWBIN, H ;
STAEHELIN, T ;
GORDON, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4350-4354
[29]  
Wassarman P.M., 1985, P341
[30]   SPERM CHYMOTRYPSIN-LIKE ENZYMES OF DIFFERENT INHIBITOR-SUSCEPTIBILITY AS LYSINS IN ASCIDIANS [J].
YOKOSAWA, H ;
NUMAKUNAI, T ;
MURAO, S ;
ISHII, S .
EXPERIENTIA, 1987, 43 (08) :925-927