STRUCTURE AND PROBABLE GENETIC LOCATION OF A RIBOSOME MODULATION FACTOR ASSOCIATED WITH 100S RIBOSOMES IN STATIONARY-PHASE ESCHERICHIA-COLI-CELLS

被引：165

作者：

WADA, A

YAMAZAKI, Y

FUJITA, N

ISHIHAMA, A

机构：

[1] NATL INST GENET, DEPT MOLEC GENET, MISHIMA, SHIZUOKA 411, JAPAN

[2] KYOTO UNIV, FAC SCI, DEPT PHYS, SAKYO KU, KYOTO 606, JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 07期

关键词：

growth-dependent control; translation regulation; two-dimensional gel electrophoresis;

D O I：

10.1073/pnas.87.7.2657

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The decrease in overall translation activity occurring concomitantly with the transition from the exponential growth phase to the stationary phase of Escherichia coli cells was found to be accompanied by the appearance of 100S ribosomes (dimers of 70S ribosome monomers). Analysis of ribosomal proteins by the radical-free and highly reducing method of two-dimensional gel electrophoresis indicated that a protein, designated protein E, was exclusively associated with 100S ribosomes. From the results, we propose that protein E is a 'ribosome modulation factor' (RMF), which associates with 70S ribosomes and converts them to a dimeric form. A homology search of the partial amino acid sequence of RMF using the DNA sequence data bases revealed that the rmf gene, which encodes RMF, is located next to the fabA gene at 21.8 min on the E. coli chromosome.

引用

页码：2657 / 2661

页数：5

共 17 条

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