REGENERATION OF PLANTS FROM LEAF MESOPHYLL PROTOPLASTS OF THE TETRAPLOID POTATO CULTIVARS XENIA AND BINTJE

被引：9

作者：

BRIZA, J

MACHOVA, I

机构：

[1] Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, České Budějovice, 370 05

来源：

BIOLOGIA PLANTARUM | 1991年 / 33卷 / 03期

关键词：

D O I：

10.1007/BF02897888

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An improved method for the isolation of leaf mesophyll protoplasts of Solanum tuberosum L. cvs. Xenia and Bintje, their culture and regeneration to plants is described. The improvements involve the culture of nodal stem explants on Schenk and Hildebrandt (SH) medium supplemented with AgNO3 (7.5 mg 1(-1)) and Alar 85 (5 mg 1(-1)), and the pre-treatment of donor plants for 2-3 weeks at a short photoperiod (6h), a low irradiance (17-mu-mol m-2 s-1) and a low temperature (20-degrees-C). Regeneration of cell wals was observed within 2-3 d and cell divisions within 7-12 d after resuspending freshly isolated protoplasts in Sidorov et al. (SW) medium. The resulting microcalli were cultured on solid media containing zeatin (1-2,5 mg 1(-1)) and/or NAA (0.1 and/or 0.01 mg 1(-1)) and subsequently on medium with BAR (0.25 mg 1(-1)) and GA3 (0.1 mg 1(-1)) for shoot regeneration.

引用

页码：225 / +

页数：1

共 19 条

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