CONSTITUTIVE EXPRESSION OF PORE-FORMING PROTEIN IN PERIPHERAL-BLOOD GAMMA-DELTA-T-CELLS - IMPLICATION FOR THEIR CYTOTOXIC ROLE INVIVO

被引：108

作者：

NAKATA, M

SMYTH, MJ

NORIHISA, Y

KAWASAKI, A

SHINKAI, Y

OKUMURA, K

YAGITA, H

机构：

[1] JUNTENDO UNIV, SCH MED, DEPT IMMUNOL, 2-1-1 HONGO, BUNKYO KU, TOKYO 113, JAPAN

[2] NCI, FREDERICK CANC RES & DEV CTR, EXPTL IMMUNOL LAB, FREDERICK, MD 21701 USA

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 1990年 / 172卷 / 06期

关键词：

D O I：

10.1084/jem.172.6.1877

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The cytotoxic activity and pore-forming protein (PFP) expression of human peripheral blood (PB) γ/δ T cells were examined. Fresh γ/δ T cells isolated from PB lymphocytes by fluorescenceactivated cell sorting exhibited a substantial natural killer-like cytotoxic activity against K562 target cells and had a high cytotoxic potential triggered by anti-CD3 monoclonal antibody (mAb) against P815 target cells bearing Fc γR. Immunocytochemical staining with an anti-PFP mAb revealed that virtually all PB γ/δ T cells are granular lymphocytes with abundant PFP in their cytoplasmic granules. Constitutive expression of PFP in PB 'y/6 T cells was also demonstrated by Northern blot analysis. These observations support the proposed role ofγ/δ T cells in cytolysic immune surveillance in vivo. © 1990, Rockefeller University Press., All rights reserved.

引用

页码：1877 / 1880

页数：4

共 16 条

[1] BRENNER MB, 1988, ADV IMMUNOL, V43, P133
[2] FALINI B, 1989, J IMMUNOL, V143, P2480
[3] FAURE F, 1988, J IMMUNOL, V140, P1372
[4] MORPHOLOGIC AND FUNCTIONAL-CHARACTERIZATION OF HUMAN PERIPHERAL-BLOOD T-CELLS EXPRESSING THE T-CELL RECEPTOR-GAMMA-DELTA
FERRINI, S
ZARCONE, D
VIALE, M
CERRUTI, G
MILLO, R
MORETTA, A
GROSSI, CE
[J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1989, 19 (07) : 1183 - 1188
[5] INTRAEPITHELIAL LYMPHOCYTES - ANATOMICAL SITE, NOT T-CELL RECEPTOR FORM, DICTATES PHENOTYPE AND FUNCTION
GOODMAN, T
LEFRANCOIS, L
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 170 (05) : 1569 - 1581
[6] HUMAN-T CELLS EXPRESSING THE GAMMA-DELTA T-CELL RECEPTOR (TCR-1) - C-GAMMA-1-ENCODED AND C-GAMMA-2-ENCODED FORMS OF THE RECEPTOR CORRELATE WITH DISTINCTIVE MORPHOLOGY, CYTOSKELETAL ORGANIZATION, AND GROWTH-CHARACTERISTICS
GROSSI, CE
CICCONE, E
MIGONE, N
BOTTINO, C
ZARCONE, D
MINGARI, MC
FERRINI, S
TAMBUSSI, G
VIALE, O
CASORATI, G
MILLO, R
MORETTA, L
MORETTA, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (05) : 1619 - 1623
[7] HOMING OF A GAMMA-DELTA THYMOCYTE SUBSET WITH HOMOGENEOUS T-CELL RECEPTORS TO MUCOSAL EPITHELIA
ITOHARA, S
FARR, AG
LAFAILLE, JJ
BONNEVILLE, M
TAKAGAKI, Y
HAAS, W
TONEGAWA, S
[J]. NATURE, 1990, 343 (6260) : 754 - 757
[8] FRONTIERS OF THE IMMUNE-SYSTEM
JANEWAY, CA
[J]. NATURE, 1988, 333 (6176) : 804 - 806
[9] PERFORIN, A PORE-FORMING PROTEIN DETECTABLE BY MONOCLONAL-ANTIBODIES, IS A FUNCTIONAL MARKER FOR KILLER-CELLS
KAWASAKI, A
SHINKAI, Y
KUWANA, Y
FURUYA, A
IIGO, Y
HANAI, N
ITOH, S
YAGITA, H
OKUMURA, K
[J]. INTERNATIONAL IMMUNOLOGY, 1990, 2 (07) : 677 - 684
[10] OSHIMI K, 1990, BLOOD, V75, P704

← 1 2 →