KINESIN-RELATED POLYPEPTIDE IS ASSOCIATED WITH VESICLES FROM CORYLUS-AVELLANA POLLEN

被引:45
作者
LIU, GQ
CAI, G
DELCASINO, C
TIEZZI, A
CRESTI, M
机构
[1] UNIV SIENA,DIPARTIMENTO BIOL AMBIENTALE,I-53100 SIENA,ITALY
[2] UNIV TUSCIA,DIPARTIMENTO SCI AMBIENTALI,VITERBO,ITALY
[3] BEIJING AGR UNIV,COLL BIOL SCI,BEIJING 100094,PEOPLES R CHINA
来源
CELL MOTILITY AND THE CYTOSKELETON | 1994年 / 29卷 / 02期
关键词
GOLGI VESICLES; POLLEN; POLLEN TUBE; MICROTUBULES; KINESIN-RELATED PROTEIN;
D O I
10.1002/cm.970290207
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A 100-kDa polypeptide with microtubule-interacting properties was identified in a Golgi vesicle-enriched fraction from Corylus avellana pollen. The k71s23 antibody (directed to the kinesin heavy chain from bovine brain) [Tiezzi et al., 1992: Cell Motil. Cytoskeleton 21:132-137] localized the polypeptide on the external surface of membrane-bounded organelles. Some 100-kDa-containing vesicles copelleted with microtubules (polymerized from purified bovine brain tubulin) either in presence or absence of 5 mM AMPPNP, but they could be released by 10 mM ATP or 0.5 M KCl. The pollen microtubule-interacting protein, salt-extracted from membranes and partially purified by gel filtration, exhibited an ATPase activity (16.2 nmolPi/mg/min) which could be stimulated about 2-fold (32.5 nmolPi/mg/min) by addition of bovine brain microtubules. We suppose that the 100-kDa polypeptide is part of a molecular complex showing properties of the kinesin class. (C) 1994 Wiley-Liss, Inc.
引用
收藏
页码:155 / 166
页数:12
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