REGULATION OF BIOSYNTHESIS OF SECONDARY METABOLITES .4. INHIBITION OF CITRATE SYNTHASE IN STREPTOMYCES AUREOFACIENS BY ADENOSINE TRIPHOSPHATE

被引：12

作者：

HOSTALEK, Z

RYABUSHKO, TA

CUDLIN, J

VANEK, Z

机构：

[1] Department of Biogenesis of Natural Substances, Institute of Microbiology, Czechoslovak Academy of Sciences

来源：

FOLIA MICROBIOLOGICA | 1969年 / 14卷 / 02期

关键词：

D O I：

10.1007/BF02892880

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Partially purified citrate synthase (EC 4.1.3.7) was isolated from submerged mycelium of Streptomyces aureofaciens RIA 57, a strain producing chlortetracycline. Enzymatic activity was determined spectrophotometrically by means of 5,5′-dithio-bis-(2-nitrobenzoic acid). Citrate synthase was inhibited by ATP, inhibition being competitive with respect to acetyl coenzyme A. In 5 mm concentration, ATP caused 55% inhibition of the enzyme. ADP, in the same concentration, caused 30% inhibition, while AMP caused none. Other natural nucleoside di- and triphosphates, in 1-5 mm concentration, did not significantly affect citrate synthase in Streptomyces aureofaciens. Mg2+ inhibited the activity of the enzyme, but also reduced the negative effect of ATP. The role of ATP in regulation of the metabolic paths of acetyl CoA is discussed. © 1969 Academia, nakladatelství Československé akademie věd.

引用

页码：121 / +

页数：1

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