CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING RHIZOPUS-NIVEUS LIPASE

被引：31

作者：

KUGIMIYA, W

OTANI, Y

KOHNO, M

HASHIMOTO, Y

机构：

[1] Central Research Institute, Fuji Oil Co., Ltd., Ibaraki 300-24, 4-3 Kinunodai, Yawara, Tsukuba-gun

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1992年 / 56卷 / 05期

关键词：

D O I：

10.1271/bbb.56.716

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complementary DNA encoding Rhizopus niveus lipase (RNL) was isolated from the R. niveus IF04759 cDNA library using a synthetic oligonucleotide corresponding to the amino acid sequence of the enzyme. A clone, which had an insert of 1.0 kilobase pairs, was found to contain the coding region of the enzyme. The lipase gene was expressed in Escherichia coli as a lacZ fusion protein. The mature RNL consisted of 297 amino acid residues with a molecular mass of 32 kDa. The RNL sequence showed significant overall homology to Rhizomucor miehei lipase and the putative active site residues were strictly conserved.

引用

页码：716 / 719

页数：4

共 19 条

[1] PURIFICATION AND PROPERTIES OF LIPASE FROM RHIZOPUS-JAPONICUS [J].