LIQUID-CHROMATOGRAPHIC ANALYSIS OF PROPRANOLOL ENANTIOMERS IN HUMAN BLOOD USING PRECOLUMN DERIVATIZATION WITH (+)-1-(9-FLUORENYL)ETHYL CHLOROFORMATE

被引：19

作者：

ROUX, A

BLANCHOT, G

BAGLIN, A

FLOUVAT, B

机构：

[1] Laboratoire de Toxicologie et de Pharmacocinétique, Hôpital Ambroise Paré, 92104 Boulogne Billancourt

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 570卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80553-O

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of the R-(+) and S-(-) enantiomers of propranolol in blood was developed. After extraction with heptane-isopentanol and derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate, excess reagent was removed using solid-phase extraction. The enantiomers were separated on an achiral, reversed-phase, radially compressed column, and detected by fluorescence with excitation and emission wavelengths of 260 and 340 nm, respectively. The limit of quantification was 0.5 ng/ml. This method was used for pharmacokinetic analysis of propranolol enantiomers after administration of immediate-release (80 mg) or sustained-release (160 mg) racemic propranolol.

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页码：453 / 461

页数：9

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