NONRADIOACTIVE SSCP FOR GENOTYPING HUMAN PLATELET ALLOANTIGENS

被引：23

作者：

PEYRUCHAUD, O ^{[1
]}

NURDEN, A ^{[1
]}

BOURRE, F ^{[1
]}

机构：

[1] HOP CARDIOL,CNRS,URA 1464,F-33604 PESSAC,FRANCE

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1995年 / 89卷 / 03期

关键词：

PLATELETS; GENOTYPING ALLOANTIGENS; GLYCOPROTEIN; IIB-IIIA COMPLEXES; PCR-SSCP;

D O I：

10.1111/j.1365-2141.1995.tb08376.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Human platelet alloantigen systems are responsible for neonatal and post-transfusional thrombocytopenias. The determination of the different allotypes can be performed using immunological or DNA-based methods. The most used DNA-based procedure requires the digestion by specific restriction enzymes of PCR products containing the genetic determinants of these alloantigens. We now report a rapid method of genotyping which does not use restriction enzymes and is less prone to misinterpretation. This is non-radioactive PCR-SSCP (single strand conformation polymorphism), which we illustrate for two different HPA systems, one on GPIIIa (HPA-1) and the other on GPIIb (HPA-3).

引用

页码：633 / 636

页数：4

共 12 条

[1] AN IMPROVED DNA-BASED IDENTIFICATION OF FETUSES AT RISK FOR HPA-1A (PL(AL)) NEONATAL ALLOIMMUNE THROMBOCYTOPENIA [J].