PRIMERS FOR MITOCHONDRIAL-DNA REPLICATION GENERATED BY ENDONUCLEASE-G

被引：197

作者：

COTE, J

RUIZCARRILLO, A

机构：

[1] HOP HOTEL DIEU,LAVAL UNIV,SCH MED,CANC RES CTR,QUEBEC CITY G1R 2J6,QUEBEC,CANADA

[2] HOP HOTEL DIEU,LAVAL UNIV,SCH MED,DEPT BIOCHEM,QUEBEC CITY G1R 2J6,QUEBEC,CANADA

来源：

SCIENCE | 1993年 / 261卷 / 5122期

关键词：

D O I：

10.1126/science.7688144

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Endonuclease G (Endo G) is widely distributed among animals and cleaves DNA at double-stranded (dG)n.(dC)n and at single-stranded (dC)n tracts. Endo G is synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria. Endo G can also be detected in extranucleolar chromatin. In addition to deoxyribonuclease activities, Endo G also has ribonuclease (RNase) and RNase H activities and specifically cleaves mouse mitochondrial RNA and DNA-RNA substrates containing the origin of heavy-strand DNA replication (O(H)). The cleavage sites match those found in vivo, indicating that Endo G is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.

引用

页码：765 / 769

页数：5