TATA BOX AND INITIATOR FUNCTIONS IN THE ACCURATE TRANSCRIPTION OF A PLANT MINIMAL PROMOTER IN-VITRO

被引:69
作者
ZHU, Q [1 ]
DABI, T [1 ]
LAMB, C [1 ]
机构
[1] SALK INST BIOL STUDIES, PLANT BIOL LAB, LA JOLLA, CA 92037 USA
关键词
D O I
10.1105/tpc.7.10.1681
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The functional architecture of the proximal region of a rice phenylalanine ammonia-lyase (PAL) promoter was analyzed by transcription of PAL-beta-glucuronidase (GUS) templates by whole-cell extracts of rice cell suspension cultures. The promoter 5' truncated to position -35 was sufficient for accurate initiation of basal transcription. Substitution of the TATTTAA sequence between positions -35 and -28 with GCGGGTT or 2-bp substitutions to give TCGTTAA and TATGGAA inactivated the minimal promoter. Moreover, the function of the TATTTAA sequence was dependent on its position relative to the initiation site; hence, this element is an authentic TATA box essential for RNA polymerase Ii-dependent transcription. Substitutions in the TCCAAG initiator cis element (-3 to +3) at the -1 (C to A or G) and +1 (A to C or T) residues caused inaccurate initiation, whereas mutations at the other residues of this conserved element or sequence substitutions between the TATA box and initiator had little effect. TATA box and initiator functions were confirmed by analysis of the effects of promoter mutations on expression in stably transformed rice cell suspensions and plants, We concluded that the proximal region of the PAL promoter has a simple functional architecture involving a TATA box appropriately positioned upstream of the initiator, Transcription of derivatives of such minimal promoters by highly active cell extracts should allow molecular analysis of functional interactions between specific cis elements and cognate trans factors.
引用
收藏
页码:1681 / 1689
页数:9
相关论文
共 51 条
[21]   AN IMPROVED RICE TRANSFORMATION SYSTEM USING THE BIOLISTIC METHOD [J].
LI, LC ;
QU, RD ;
DEKOCHKO, A ;
FAUQUET, C ;
BEACHY, RN .
PLANT CELL REPORTS, 1993, 12 (05) :250-255
[22]   RNA POLYMERASE-II INITIATION-FACTOR INTERACTIONS AND TRANSCRIPTION START SITE SELECTION [J].
LI, Y ;
FLANAGAN, PM ;
TSCHOCHNER, H ;
KORNBERG, RD .
SCIENCE, 1994, 263 (5148) :805-807
[23]   MECHANISM OF ACTION OF AN ACIDIC TRANSCRIPTIONAL ACTIVATOR INVITRO [J].
LIN, YS ;
GREEN, MR .
CELL, 1991, 64 (05) :971-981
[24]   TATA-BINDING PROTEIN-ASSOCIATED FACTOR(S) IN TFIID FUNCTION THROUGH THE INITIATOR TO DIRECT BASAL TRANSCRIPTION FROM A TATA-LESS CLASS-II PROMOTER [J].
MARTINEZ, E ;
CHIANG, CM ;
GE, H ;
ROEDER, RG .
EMBO JOURNAL, 1994, 13 (13) :3115-3126
[25]   THE PITUITARY-SPECIFIC REGULATORY GENE GHF1 CONTAINS A MINIMAL CELL TYPE-SPECIFIC PROMOTER CENTERED AROUND ITS TATA BOX [J].
MCCORMICK, A ;
BRADY, H ;
FUKUSHIMA, J ;
KARIN, M .
GENES & DEVELOPMENT, 1991, 5 (08) :1490-1503
[26]   TRANSCRIPTION INITIATION FROM THE DIHYDROFOLATE-REDUCTASE PROMOTER IS POSITIONED BY HIP1 BINDING AT THE INITIATION SITE [J].
MEANS, AL ;
FARNHAM, PJ .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (02) :653-661
[27]   NUCLEOTIDE-SEQUENCE OF THE GENE FOR PHENYLALANINE AMMONIA-LYASE OF RICE AND ITS DEDUCED AMINO-ACID-SEQUENCE [J].
MINAMI, E ;
TANAKA, Y .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1171 (03) :321-322
[28]   STRUCTURE AND SOME CHARACTERIZATION OF THE GENE FOR PHENYLALANINE AMMONIA-LYASE FROM RICE PLANTS [J].
MINAMI, E ;
OZEKI, Y ;
MATSUOKA, M ;
KOIZUKA, N ;
TANAKA, Y .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 185 (01) :19-25
[29]   A SHORT CONSERVED SEQUENCE IS INVOLVED IN THE LIGHT-INDUCIBILITY OF A GENE ENCODING RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE SMALL SUBUNIT OF PEA [J].
MORELLI, G ;
NAGY, F ;
FRALEY, RT ;
ROGERS, SG ;
CHUA, NH .
NATURE, 1985, 315 (6016) :200-204
[30]   THE 30-KDA PROTEIN-BINDING TO THE INITIATOR OF THE BACULOVIRUS POLYHEDRIN PROMOTER ALSO BINDS SPECIFICALLY TO THE CODING STRAND [J].
MUKHERJEE, B ;
BURMA, S ;
HASNAIN, SE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (09) :4405-4411