LONG-RANGE ANALYSES OF THE CENTROMERIC REGIONS OF HUMAN CHROMOSOME-13, CHROMOSOME-14 AND CHROMOSOME-21 - IDENTIFICATION OF A NARROW DOMAIN-CONTAINING 2 KEY CENTROMERIC DNA ELEMENTS

被引：80

作者：

TROWELL, HE ^{[1
]}

NAGY, A ^{[1
]}

VISSEL, B ^{[1
]}

CHOO, KHA ^{[1
]}

机构：

[1] ROYAL CHILDRENS HOSP,MURDOCH INST RES BIRTH DEFECTS,FLEMINGTON RD,PARKVILLE,VIC 3052,AUSTRALIA

来源：

HUMAN MOLECULAR GENETICS | 1993年 / 2卷 / 10期

关键词：

D O I：

10.1093/hmg/2.10.1639

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alpha-satellite, satellite 3 and satellite 1 DNA have been proposed as candidate components of a functional human centromere. Multiple subfamilies of these three DNA have recently been identified at the pericentric regions of the human acrocentric chromosomes. Using pulsed field gel electrophoresis, we have constructed long-range maps of the various centromeric markers for chromosomes 13, 14 and 21. These maps cover approximately 2.3 megabases of sequence for each chromosome, and the results demonstrate that within this centromeric region, chromosomes 13 and 21 have a similar organization that is partially shared by chromosome 14. A discrete satellite 3 domain was identified on each chromosome within the boundaries of the alpha-satellite DNA. No satellite 1 was detected within the defined centromeric regions suggesting that satellite 1 is not essential for centromere function.

引用

页码：1639 / 1649

页数：11

共 28 条

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