MONOCLONAL-ANTIBODY C242-PSEUDOMONAS EXOTOXIN-A - A SPECIFIC AND POTENT IMMUNOTOXIN WITH ANTITUMOR-ACTIVITY ON A HUMAN COLON CANCER XENOGRAFT IN NUDE-MICE

被引：36

作者：

DEBINSKI, W ^{[1
]}

KARLSSON, B ^{[1
]}

LINDHOLM, L ^{[1
]}

SIEGALL, CB ^{[1
]}

WILLINGHAM, MC ^{[1
]}

FITZGERALD, D ^{[1
]}

PASTAN, I ^{[1
]}

机构：

[1] PHARM CANAG, S-40242 GOTHENBURG, SWEDEN

来源：

JOURNAL OF CLINICAL INVESTIGATION | 1992年 / 90卷 / 02期

关键词：

COLON CANCER; IMMUNOTOXINS; PSEUDOMONAS EXOTOXIN-A; SOLID TUMOR THERAPY;

D O I：

10.1172/JCI115875

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Two immunotoxins were constructed by chemically coupling the monoclonal antibody C242 to Pseudomonas exotoxin A (PE) or a modified form, NlysPE40, that lacks the cell binding domain of PE. Monoclonal antibody C242 recognizes a specific sialylated carbohydrate epitope on a high molecular weight membrane glycoprotein present on cells of human colon, pancreatic, and cervical cancers. C242-PE and C242-NlysPE40 were very cytotoxic for cells expressing this antigen with 50% inhibition of protein synthesis occurring on Colo205 cells at 0.2 ng/ml (0.9 pM) for C242-PE and 6.0 ng/ml (31 pM) for C242-NlysPE40. The two immunotoxins also exhibited a strong antitumor effect on a human colon cancer xenograft grown in nude mice. The specificity and potency of these two C242 immunotoxins warrant their further development for the treatment of cancer.

引用

页码：405 / 411

页数：7