REINVESTIGATION OF THE THERMODYNAMICS OF SPECTRIN SELF-ASSOCIATION

被引:11
作者
HENNIKER, A [1 ]
RALSTON, GB [1 ]
机构
[1] UNIV SYDNEY,DEPT BIOCHEM,SYDNEY,NSW 2006,AUSTRALIA
基金
澳大利亚研究理事会;
关键词
SPECTRIN; SEDIMENTATION EQUILIBRIUM; ULTRACENTRIFUGE; THERMODYNAMICS; HYDROPHOBIC INTERACTIONS; VANT HOFF;
D O I
10.1016/0301-4622(94)00094-Z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The thermodynamics of the self-association reactions of human spectrin have been reinvestigated by means of sedimentation equilibrium over the temperature range 18-40 degrees C. The experimental data were analysed in terms of a cooperative isodesmic model of association. The van't Hoff plot showed that the standard change in enthalpy for the heterodimer-tetramer step was temperature-dependent, leading to an estimate of -8.5 kJ mol(-1) K-1 for the change in molar heat capacity, Delta C-p. Curvature in the van't Hoff plots, not detected in previous studies, was revealed through the increased precision of the data and the wider temperature range examined. On the assumption that Delta C-p reflects hydrophobic interactions in the tetramer that cannot be formed in the heterodimer, it can be estimated that approximately 50 CH2 groups per heterodimer participate in hydrophobic interactions in the tetramer that cannot be formed in the heterodimer.
引用
收藏
页码:251 / 258
页数:8
相关论文
共 23 条
[11]   THE USE OF METRIZAMIDE FOR STABILIZING AGAINST CONVECTION IN SEDIMENTATION EQUILIBRIUM [J].
RALSTON, GB ;
TELLER, DC ;
BUKOWSKI, T .
ANALYTICAL BIOCHEMISTRY, 1989, 178 (01) :198-201
[12]  
RALSTON GB, 1991, BIOCHEMISTRY-US, V30, P3076
[13]  
RALSTON GB, 1992, ANAL ULTRACENTRIFUGA, P253
[14]  
SAHR KE, 1990, J BIOL CHEM, V265, P4434
[15]   ANALYSIS OF THE SELF-ASSOCIATION OF HUMAN RED-CELL SPECTRIN [J].
SHAHBAKHTI, F ;
GRATZER, WB .
BIOCHEMISTRY, 1986, 25 (20) :5969-5975
[16]   MOLECULAR-STRUCTURE OF HUMAN-ERYTHROCYTE SPECTRIN - BIOPHYSICAL AND ELECTRON-MICROSCOPIC STUDIES [J].
SHOTTON, DM ;
BURKE, BE ;
BRANTON, D .
JOURNAL OF MOLECULAR BIOLOGY, 1979, 131 (02) :303-329
[17]  
SPEICHER DW, 1993, J BIOL CHEM, V268, P4227
[18]  
Tanford C, 1970, Adv Protein Chem, V24, P1, DOI 10.1016/S0065-3233(08)60241-7
[19]  
Teller D C, 1973, Methods Enzymol, V27, P346
[20]  
TYLER JM, 1979, P NATL ACAD SCI USA, V76, P76