PURIFICATION AND CHARACTERIZATION OF A NEUROTOXIC PHOSPHOLIPASE-A2 FROM INDIAN COBRA (NAJA-NAJA-NAJA) VENOM

被引：24

作者：

BHAT, MK ^{[1
]}

PRASAD, BN ^{[1
]}

GOWDA, TV ^{[1
]}

机构：

[1] UNIV MYSORE,DEPT STUDIES BIOCHEM,MYSORE 570006,KARNATAKA,INDIA

来源：

TOXICON | 1991年 / 29卷 / 11期

关键词：

D O I：

10.1016/0041-0101(91)90121-7

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Snake venoms contain multimolecular forms of phospholipase A2 which are diverse with respect to their pharmacological properties. A neurotoxic PLA2 from Naja naja naja venom has been purified in two steps. (1) The whole venom was fractionated on CM-Sephadex C-25 column; 4.6% of the total PLA2 activity recovered was found in the NN-V fraction. (2) The NN-Vb-PLA2 fraction was purified to homogeneity by gel filtration of fraction NN-V on Sephadex G-50. It is a basic protein with a mol. wt between 10,500 and 11,000, and is more toxic than other basic PLA2s purified from Naja naja naja venom. The LD50 Of NN-Vb-PLA2 is 0.27 mg/kg body wt. It induced neurotoxic symptoms in experimental mice and is devoid of myotoxic, anticoagulant and edema-inducing activities.

引用

页码：1345 / 1349

页数：5

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