CALCIUM INFLUX THROUGH ANNEXIN-V ION CHANNELS INTO LARGE UNILAMELLAR VESICLES MEASURED WITH FURA-2

被引：54

作者：

BERENDES, R

BURGER, A

VOGES, D

DEMANGE, P

HUBER, R

机构：

[1] Max-Planck-Institut für Biochemie, 8033 Martinsried, Am Klopferspitz

来源：

FEBS LETTERS | 1993年 / 317卷 / 1-2期

关键词：

ANNEXIN-V; CALCIUM CHANNEL; LIPOSOME; FURA-2;

D O I：

10.1016/0014-5793(93)81507-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V.

引用

收藏

页码：131 / 134

页数：4

相关论文

共 26 条

[1] AMINO-ACID-SEQUENCE ANALYSIS OF THE ANNEXIN SUPERGENE FAMILY OF PROTEINS [J].

BARTON, GJ ;

NEWMAN, RH ;

FREEMONT, PS ;

CRUMPTON, MJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 198 (03) :749-760

[2] TRANSMEMBRANE CALCIUM MOVEMENTS MEDIATED BY IONOMYCIN AND PHOSPHATIDATE IN LIPOSOMES WITH FURA-2 ENTRAPPED [J].

BLAU, L ;

WEISSMANN, G .

BIOCHEMISTRY, 1988, 27 (15) :5661-5666

[3] THE STOICHIOMETRY OF A23187-MEDIATED AND X537A-MEDIATED CALCIUM-ION TRANSPORT ACROSS LIPID BILAYERS [J].

BLAU, L ;

STERN, RB ;

BITTMAN, R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 778 (01) :219-223

[4] THE ANNEXINS AND EXOCYTOSIS [J].

CREUTZ, CE .

SCIENCE, 1992, 258 (5084) :924-931

[5] DIVERSITY IN THE LIPOCORTIN CALPACTIN FAMILY [J].

CROMPTON, MR ;

MOSS, SE ;

CRUMPTON, MJ .

CELL, 1988, 55 (01) :1-3

[6]

DAVIDSON FF, 1990, J BIOL CHEM, V265, P5602

[7] IDENTIFICATION OF A CELLULAR PROTEIN SUBSTRATE PHOSPHORYLATED BY THE AVIAN-SARCOMA VIRUS-TRANSFORMING GENE-PRODUCT [J].

ERIKSON, E ;

ERIKSON, RL .

CELL, 1980, 21 (03) :829-836

[8]

GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440

[9] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].

HAMILL, OP ;

MARTY, A ;

NEHER, E ;

SAKMANN, B ;

SIGWORTH, FJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100

[10] CRYSTAL AND MOLECULAR-STRUCTURE OF HUMAN ANNEXIN-V AFTER REFINEMENT - IMPLICATIONS FOR STRUCTURE, MEMBRANE-BINDING AND ION CHANNEL FORMATION OF THE ANNEXIN FAMILY OF PROTEINS [J].

HUBER, R ;

BERENDES, R ;

BURGER, A ;

SCHNEIDER, M ;

KARSHIKOV, A ;

LUECKE, H ;

ROMISCH, J ;

PAQUES, E .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 223 (03) :683-704