CALCIUM INFLUX THROUGH ANNEXIN-V ION CHANNELS INTO LARGE UNILAMELLAR VESICLES MEASURED WITH FURA-2

被引：54

作者：

BERENDES, R

BURGER, A

VOGES, D

DEMANGE, P

HUBER, R

机构：

[1] Max-Planck-Institut für Biochemie, 8033 Martinsried, Am Klopferspitz

来源：

FEBS LETTERS | 1993年 / 317卷 / 1-2期

关键词：

ANNEXIN-V; CALCIUM CHANNEL; LIPOSOME; FURA-2;

D O I：

10.1016/0014-5793(93)81507-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V.

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页码：131 / 134

页数：4

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